Fluorescence lifetime imaging microscopy (FLIM): a non-traditional approach to study host-microbial symbioses

P Deore, I Wanigasuriya, SJTM Ching… - Microbiology …, 2022 - CSIRO Publishing
… ) coupled with fluorescence lifetime imaging microscopy (FLIM… in situ location and identity
of bacteria in Symbiodiniaceae. … endogenous chemicals (eg metabolic co-enzymes, structural …

[HTML][HTML] Raman imaging and fluorescence lifetime imaging microscopy for diagnosis of cancer state and metabolic monitoring

L Becker, N Janssen, SL Layland, TE Muerdter… - Cancers, 2021 - mdpi.com
… -active [8] and thus provide molecular fingerprints that are highly sensitive and can reflect a
… modalities of these techniques for cancer imaging, metabolic monitoring, and diagnosis. In …

[PDF][PDF] Full-spectrum fluorescence lifetime imaging microscopyprofiling photochemical properties of fluorophores in their microenvironment

Z Konieczna, G Williams, M Horrocks - Biophysical Journal, 2022 - cell.com
… ) play an important role in bacterial metabolism and cellular processes as well … bacterial
cells as well as in bacterial communities are limited. A fluorescence lifetime imaging microscopy (…

[HTML][HTML] Real-time, label-free detection and identification of bacteria through non-invasive optical imaging

F Qazi, R Verma, CE Redmond, A Khalid… - Microbes and …, 2024 - Elsevier
… spectra and fluorescence lifetimes can be used as a fingerprint for … fluorescence lifetime to
distinguish bacteria from each other. … We hypothesised that the metabolic differences between …

[HTML][HTML] Microglia activation visualization via fluorescence lifetime imaging microscopy of intrinsically fluorescent metabolic cofactors

MAK Sagar, JN Ouellette, KP Cheng… - …, 2020 - spiedigitallibrary.org
… free signatures for different cell types ranging from bacteria to stem cells. A strategy reported
… that mean NADH lifetime is well-suited for identifying a microglial fingerprint upon activation. …

A rapid analysis platform for investigating the cellular locations of bacteria using two-photon fluorescence lifetime imaging microscopy

N Sapermsap, DDU Li, R Al-Hemedawi… - … in Fluorescence, 2020 - iopscience.iop.org
… , a red-fluorescent by-product of bacterial haem production, and … Most interestingly, two-photon
FLIM imaging of the metabolic … been used for bacterial metabolic fingerprinting in diverse …

Label-free multiphoton imaging of microbes in root, mineral, and soil matrices with time-gated coherent Raman and fluorescence lifetime imaging

J Lee, R Hestrin, EE Nuccio, KD Morrison… - … science & technology, 2022 - ACS Publications
… for biomedical imaging and studies of cellular metabolism due … of imaging fluorescently
labeled bacteria, we imaged DAPI-… In future work, CARS bands from the so-called “fingerprint” …

Single cell fluorescence imaging of glycan uptake by intestinal bacteria

JH Hehemann, G Reintjes, L Klassen… - The ISME …, 2019 - academic.oup.com
… degradation and transport were non-fluorescent. Uptake of FGCs, … metabolism in intestinal
bacteria at the single cell level. … selected for fluorescence intensity line profiling are indicated …

In vivo fluorescence lifetime imaging captures metabolic changes in macrophages during wound responses in zebrafish

V Miskolci, KE Tweed, MR Lasarev, EC Britt… - bioRxiv, 2020 - biorxiv.org
… the bacterial metabolic data (see figure legend and methods) to exclude from macrophage
profiling. L monocytogenes infection of macrophages resulted in the increase of the mean …

[HTML][HTML] Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms

A Battisti, P Morici, A Sgarbossa - Pharmaceutics, 2021 - mdpi.com
… in the image to a point in the phasor plot [15]. The FLIM-phasor approach has been used to
… a metabolic fingerprint of individual bacteria and pop-ulations by monitoring the lifetime of …